AgrobacTransV1, Main, Exploration, bibRecord, 000368

Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.

Identifieur interne : 000368 ( Main/Exploration ); précédent : 000367; suivant : 000369

Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.

Auteurs : Su-Li Wu [République populaire de Chine] ; Xiao-Bing Yang ; Li-Qun Liu ; Tao Jiang ; Hai Wu ; Chao Su ; Yong-Hua Qian ; Feng Jiao

Source :

Bioscience, biotechnology, and biochemistry [ 1347-6947 ] ; 2015.

RBID : pubmed:26024368

Descripteurs français

English descriptors

KwdEn :
- Agrobacterium tumefaciens (genetics), Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis Proteins (genetics), Fruit (genetics), Gene Expression Regulation, Plant (MeSH), Morus (genetics), Morus (growth & development), Plant Leaves (genetics), Plant Leaves (growth & development), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plasmids (MeSH), Seedlings (genetics).
MESH :
- chemical , genetics : Arabidopsis Proteins.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Fruit, Morus, Plant Leaves, Plants, Genetically Modified, Seedlings.
- growth & development : Arabidopsis, Morus, Plant Leaves, Plants, Genetically Modified.
- Gene Expression Regulation, Plant, Plasmids.

Abstract

To optimize Agrobacterium-mediated transient transformation assay in mulberry (Morus alba L.), various infiltration methods, Agrobacterium tumefaciens (A. tumefaciens) strains, and bacterial concentrations were tested in mulberry seedlings. Compared with LBA4404, GV3101 harboring pBE2133 plasmids presented stronger GUS signals at 3 days post infiltration using syringe. Recombinant plasmids pBE2133:GFP and pBE2133:GFP:MaFT were successfully constructed. Transient expression of MaFT:GFP protein was found in leaves, petiole (cross section), and shoot apical meristem (SAM) of mulberry according to the GFP signal. Moreover, MaFT:GFP mRNA was also detected in leaves and SAM via RT-PCR and qRT-PCR. An efficient transient transformation system could be achieved in mulberry seedlings by syringe using A. tumefaciens GV3101 at the OD600 of 0.5. The movement of MaFT expression from leaves to SAM might trigger the precocious flowering of mulberry.

DOI: 10.1080/09168451.2015.1025691
PubMed: 26024368

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000337
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Le document en format XML

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<author><name sortKey="Wu, Su Li" sort="Wu, Su Li" uniqKey="Wu S" first="Su-Li" last="Wu">Su-Li Wu</name>
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<term>Arabidopsis (genetics)</term>
<term>Arabidopsis (growth & development)</term>
<term>Arabidopsis Proteins (genetics)</term>
<term>Fruit (genetics)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Morus (genetics)</term>
<term>Morus (growth & development)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (growth & development)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Plasmids (MeSH)</term>
<term>Seedlings (genetics)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term>
<term>Arabidopsis (croissance et développement)</term>
<term>Arabidopsis (génétique)</term>
<term>Feuilles de plante (croissance et développement)</term>
<term>Feuilles de plante (génétique)</term>
<term>Fruit (génétique)</term>
<term>Morus (croissance et développement)</term>
<term>Morus (génétique)</term>
<term>Plant (génétique)</term>
<term>Plasmides (MeSH)</term>
<term>Protéines d'Arabidopsis (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Végétaux génétiquement modifiés (croissance et développement)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
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<term>Végétaux génétiquement modifiés</term>
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<term>Plants, Genetically Modified</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term>
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<term>Feuilles de plante</term>
<term>Fruit</term>
<term>Morus</term>
<term>Plant</term>
<term>Protéines d'Arabidopsis</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Plasmids</term>
</keywords>
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<term>Régulation de l'expression des gènes végétaux</term>
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<front><div type="abstract" xml:lang="en">To optimize Agrobacterium-mediated transient transformation assay in mulberry (Morus alba L.), various infiltration methods, Agrobacterium tumefaciens (A. tumefaciens) strains, and bacterial concentrations were tested in mulberry seedlings. Compared with LBA4404, GV3101 harboring pBE2133 plasmids presented stronger GUS signals at 3 days post infiltration using syringe. Recombinant plasmids pBE2133:GFP and pBE2133:GFP:MaFT were successfully constructed. Transient expression of MaFT:GFP protein was found in leaves, petiole (cross section), and shoot apical meristem (SAM) of mulberry according to the GFP signal. Moreover, MaFT:GFP mRNA was also detected in leaves and SAM via RT-PCR and qRT-PCR. An efficient transient transformation system could be achieved in mulberry seedlings by syringe using A. tumefaciens GV3101 at the OD600 of 0.5. The movement of MaFT expression from leaves to SAM might trigger the precocious flowering of mulberry.</div>
</front>
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<DateCompleted><Year>2016</Year>
<Month>04</Month>
<Day>26</Day>
</DateCompleted>
<DateRevised><Year>2015</Year>
<Month>07</Month>
<Day>21</Day>
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<Title>Bioscience, biotechnology, and biochemistry</Title>
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<ArticleTitle>Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.</ArticleTitle>
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<Abstract><AbstractText>To optimize Agrobacterium-mediated transient transformation assay in mulberry (Morus alba L.), various infiltration methods, Agrobacterium tumefaciens (A. tumefaciens) strains, and bacterial concentrations were tested in mulberry seedlings. Compared with LBA4404, GV3101 harboring pBE2133 plasmids presented stronger GUS signals at 3 days post infiltration using syringe. Recombinant plasmids pBE2133:GFP and pBE2133:GFP:MaFT were successfully constructed. Transient expression of MaFT:GFP protein was found in leaves, petiole (cross section), and shoot apical meristem (SAM) of mulberry according to the GFP signal. Moreover, MaFT:GFP mRNA was also detected in leaves and SAM via RT-PCR and qRT-PCR. An efficient transient transformation system could be achieved in mulberry seedlings by syringe using A. tumefaciens GV3101 at the OD600 of 0.5. The movement of MaFT expression from leaves to SAM might trigger the precocious flowering of mulberry.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Su-Li</ForeName>
<Initials>SL</Initials>
<AffiliationInfo><Affiliation>Institute of Sericulture and Silk , Northwest A&F University, Yangling, P.R. China. fjao@nwsuaf.edu.cn</Affiliation>
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<Author ValidYN="Y"><LastName>Jiao</LastName>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName>
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</MeshHeading>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MaFT</Keyword>
<Keyword MajorTopicYN="N">agro-infiltration</Keyword>
<Keyword MajorTopicYN="N">mulberry</Keyword>
<Keyword MajorTopicYN="N">transient expression</Keyword>
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<name sortKey="Liu, Li Qun" sort="Liu, Li Qun" uniqKey="Liu L" first="Li-Qun" last="Liu">Li-Qun Liu</name>
<name sortKey="Qian, Yong Hua" sort="Qian, Yong Hua" uniqKey="Qian Y" first="Yong-Hua" last="Qian">Yong-Hua Qian</name>
<name sortKey="Su, Chao" sort="Su, Chao" uniqKey="Su C" first="Chao" last="Su">Chao Su</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Wu, Su Li" sort="Wu, Su Li" uniqKey="Wu S" first="Su-Li" last="Wu">Su-Li Wu</name>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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Serveur d'exploration sur l'agrobacterium et la transgénèse

Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.

Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki